Development of Digested Sludge-Assimilating and Biohydrogen-Yielding Microflorae

Digested sludge (DS) is a waste product of anaerobic digestion that produced during the biodegradation excess sludge. It cannot be used as substrate for further biogas production owing to its recalcitrant nature. In present study, we heat treatment technique convert DABYS microflora (DABYS = digested sludge-assimilating and biogas-yielding soil microflora), which degraded DS methane gas, could produce hydrogen gas from DS. Heat at 80 100 °C inactivated methanogens consume but did not affect thermotolerant bacteria. We developed three microflorae (DABYS-A80, DABYS-A100, DABYS-80B) exclusively gas. They included representatives eubacterial families Clostridiaceae Enterobacteriaceae. Pseudomonas sp. was also in DABYS-A80 DABYS-A100. thought bacteria Enterobacteriaceae family or genus survive because they are embedded microgranules. Enzymatic analysis suggested hydrolyzed using cellulase, chitinase, protease. Under optimum culture conditions, DABYS-A80, -A100, B-100 yields 8.0, 7.1, 2.6 mL, respectively, 1.0 g dried